DirecTaq DNA polymerase saves time and cost by enabling direct PCR amplification of unpurified templates. DirecTaq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98 °C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Lacks exonuclease activity
Thermotolerant up to 98 °C
Resistant to inhibitors, e.g., whole
blood
Ideal for GC-rich templates
Buffer composition: 50% glycerol, 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA,
0.5% Tween-20, 0.5% NP-40 substitute, pH 7.5
10X DirecTaq buffer, 4 M Betaine, and
30% Sucrose included
Storage temperature: -20 °C
Options Amplified
Specificity • Sensitivity • Multiplexity
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