ReleaseIt β-Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion.
ReleaseIt β-Agarase has higher thermostability than other commercially available β-agarases, with a broad range of activity between 42 °C and 50 °C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.
Bulk β-Agarase is available (contact us for details).
Benefits
• Complete digestion of agarose, with no agarose fragments left after the digestion
• Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Concentration: 1000 u/ml
Storage buffer: 50 mM Tris-HCI, pH 7.5 + 50 mM KCI + 1 mM DTT + 0.1 mM EDTA + 50% glycerol
Operating temperature: 42 °C to 50 °C
Recommended temperature: 50 °C
Storage temperature: -20 °C
Options Amplified
Specificity • Sensitivity • Multiplexity
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