MOLECULAR DIAGNOSTICS
Fast. Simple. Accurate.
Rapid, easy-to-use and portable diagnostic assays are critical to making point-of-care and field-based testing more accessible.
TORCH™ mLAMP. A Faster and Easier Way to Test at the Point-of-Care.
Fast
Simple
Accurate
Economical
What is TORCH™ mLAMP?
Multiplex-Loop-mediated Isothermal AMPlification: A Better Way to Test.
TORCH multiplex LAMP (TORCH mLAMP) is a new way to rapidly and accurately test for several pathogens simultaneously, with minimal cost, equipment and expertise.
Traditional LAMP technology is a good alternative to PCR for point-of-care and field testing due to its speed, but it typically detects only one target per reaction. In contrast, real-time PCR can detect 3-4 targets simultaneously. This limitation in LAMP is due to its non-sequence-specific detection methods.
Common LAMP detection methods like turbidity, dyes, and gel electrophoresis are sequence-independent, limiting multi-target detection in a single tube. Lateral flow strips and melt analysis can detect multiple targets but require extra steps or specialized equipment.
At Varizymes, we have developed an innovative approach, TORCH mLAMP (patent pending), to detect multiple targets in a single LAMP reaction. Now, get all the benefits of LAMP with the ability to test for up to 4 targets per sample. TORCH multiplex LAMP (multiplex loop-mediated isothermal amplification, or mLAMP) is a simple, fast and economical way to test for several pathogens simultaneously.
For field-based testing, ease-of-use and portability are key. Combine unpurified sample with our pre-made, lyophilized master mix and test. A portable, battery-operated fluorometer will provide unambiguous results in a few minutes, onsite. There is no need to send samples to a reference lab. Samples can often be tested as is, without the need for time-consuming nucleic acid extraction. Our tests are as specific and sensitive as qPCR, but far faster and simpler to run.
Transforming Human and Animal Diagnostics
Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets
Advantages of TORCH™ mLAMP Technology
Nucleic acid extraction usually not required.
Uses standard LAMP primer design.
Unlike other mLAMP methods, TORCH does not require additional primers, probes, or extra steps such as melt analysis.
Real-time detection of amplification products.
Detection of up to 4 targets in a single mLAMP reaction (very similar to qPCR).
Similar speed, sensitivity, and specificity to a traditional LAMP assay.
Works for both DNA and RNA targets.
Multiplexing can result in significant cost savings and improved testing efficiency.
How Does TORCH mLAMP Work?
Highly Sensitive and Accurate Fluorescent Detection of up to 4 Targets
This is a TORCH mLAMP workflow for a quadruplex assay. Sample is collected and placed in a tube containing a lyophilized master mix. In most cases, no nucleic acid extraction is necessary.
Reaction mix is incubated at a constant temperature — a thermal cycler is not required. Factorial amplification occurs via 6 primers (colored bars) that bind uniquely to 6-8 distinct regions of the target.
Multiple rounds of strand displacement followed by primer annealing and polymerization form dumbell-like loop structures. These loop structures facilitate subsequent rounds of amplification producing very long (>20 kb) DNA concatamers with numerous repeats of the target sequence.
Amplification is factorial, producing detectable levels of amplified targets within 15-25 minutes. This occurs simultaneously for up to 4 targets in the same reaction. The entire process, from sample collection to results, takes less than 30 minutes and is all done on-site, at the point-of-care.
