MOLECULAR TOOLS
Accelerate Discovery.

Hot springs harbor microorganisms that produce enzymes capable of functioning at high temperatures, like DirecTaq™ DNA Polymerase.

Catalyzing Discoveries with
Precision Enzyme Solutions

We have decades of experience engineering and optimizing enzymes for improved performance. From PCR to NGS to isothermal amplification and more, Varizymes is your trusted source for quality enzymes and reagents that will accelerate your next discovery.

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NGS

Faster, More Efficient NGS Library Prep

Next-generation sequencing is a workhorse for almost every life sciences lab. However, library prep can be tedious and time consuming.

Tagmentation utilizes a transposase to simultaneously fragment DNA and add adapter sequences in a single step. This process combines DNA fragmentation and adapter ligation, which are traditionally separate steps in library preparation.

Varizymes' SwifTag™ Tn5 2.0 Transposase is a hyperactive retroviral integrase engineered for improved activity and stability. Accelerate your next sequencing experiment with SwifTag Tn5 2.0 Transposase.

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RNA

Reducing RNA Degradation

RNase contamination represents a significant and persistent threat to the quality and reliability of RNA samples. RNases are ubiquitous, found in human skin, dust, and laboratory environments, making it challenging to maintain an RNase-free workspace.

To prevent contamination, it is essential to employ rigorous decontamination techniques and use RNase inhibitors during RNA isolation and analysis.

Varizymes RNase Inhibitor is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity.

Reduce RNA degradation with Varizymes' RNase Inhibitor.

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PCR

Reliable PCR From Unpurified Templates

PCR typically requires very clean template to perform well. At Varizymes, we have expanded the reach of PCR by engineering a polymerase that is robust, even in inhibitor-rich samples, like whole blood.

Varizymes' DirecTaq™ DNA Polymerase saves time and money by enabling direct PCR amplification of unpurified templates. DirecTaq DNA Polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase isolated from the thermophilic bacterium Thermus aquaticus.

Simplify your PCR workflow by switching to DirecTaq DNA Polymerase.

APPLICATION SPOTLIGHT: TAGMENTATION

Tn5 Transposase-Based Tagmentation Enables Fast and Easy NGS Library Preparation.

Tagmentation, a revolutionary method in DNA library preparation, harnesses the unique capabilities of Tn5 transposase to simultaneously fragment and tag genomic DNA with sequencing adapters.

Why Tagmentation?


Tagmentation offers many advantages over other NGS library preparation methods.

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Fast and Simple: Streamlined Workflow Saves You Time.

  • Tn5 tagmentation enables fast, robust, and highly efficient processing of DNA samples.
  • Tn5 tagmentation allows for a streamlined, one-tube library construction protocol that is more rapid (within 15 minutes) and convenient compared to traditional multi-step methods.
  • The tagmentation process combines DNA fragmentation and adapter ligation into a single step.
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Low Input: Very low input requirement saves precious sample.

  • Tagmentation can work with very low input amounts of DNA — as little as 100-200 pg.
  • This makes it suitable for applications with limited starting material, such as single-cell genomics.
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Versatile: Adapted for many applications.

  • Tn5 tagmentation has been adapted for various applications beyond standard DNA sequencing, including RNAseq, ATAC-seq (for chromatin accessibility profiling) and single-cell genomics assays.
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Economical and Scalable: Save money and efficiently scale to hundreds of samples.

  • In-house library prep using Varizymes' SwifTag Tn5 2.0 Transposase can reduce library preparation costs by up to 70-fold compared to commercial kits.
  • The efficiency and simplicity of tagmentation make it well-suited for high-throughput processing of large numbers of samples in parallel.

How Does Tagmentation Work?

Tn5 transposase is combined with adapter sequences (blue and green). The adapters contain a 19-bp double-stranded Mosaic End sequence recognized by Tn5 transposase. Adapters have 5' overhangs on the transfer strand with forward or reverse sequences for subsequent PCR.

Tn5 transposase cleaves the target DNA at random locations and simultaneously ligates adapter sequences to the exposed ends.

Tagmented DNA is amplified using primers complementary to the adapter sequences. This step enriches for properly tagmented fragments and adds full sequencing adapters. The amplified library is purified to remove excess primers and adapters and is sent to the sequencer.

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Choose From Our Growing Portfolio of Molecular Tools

ENZYMES

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neoBolt™ Bst DNA Polymerase
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SwifTag™ Tn5 2.0 Transposase
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TEV Protease
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ReleaseIt™ β-Agarase
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Cod Uracil-DNA Glycosylase
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CRISPR/Cas9 Endonucleases
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Thunder™ Reverse Transcriptase
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EconoScript™ Reverse Transcriptase
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DirecTaq™ DNA Polymerase
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DirecTaq™ 1X Master Mix

PROTEINS

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RNase Inhibitor
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T4 Gene 32 Protein

CONTROLS

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MS2 Phage
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VariSafe™ RNA Controls

What Our Customers Are Saying

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We got outstanding sequencing results when using Varizymes SwifTag™ Tn5 2.0 as the first step of our NGS library prep.



Compared to samples prepared with an enzymatic fragmentation method, followed by end-repair and adapter ligation (prepped at the same time and included in the same sequencing run), direct incorporation of the adapter using SwifTag Tn5 produced higher mapping efficiency, sensitivity, and reproducibility.

Jesse Riordan, University of Iowa